Post-Doctoral Research Fellow, Comprehensive Discovery of HIV Latency Factors - Fred Hutch
Description:
Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer with minimal side effects. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first and largest cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
The Emerman lab was awarded an Avant-Garde Award from NIDA to study HIV latency. We have developed a novel screening technology called HIV-CRISPR technology to comprehensively discover the pathways of HIV latency maintenance and establishment. This postdoctoral position will further explore HIV latency by developing new variations of these CRISPR screens and by validating and further exploring mechanisms of the hits. Preference for new PhD with expertise in one of the following areas: chromatin biology, transcription factors, primary T cell culture, or models of HIV latency. Expectation is to develop an independent project within the context of the grant. The responsibilities include those expected at a PhD level, including leading the technical aspects of the study, contributing intellectually to the overall study design, interpreting data, presenting the findings at meetings and writing manuscripts.
We are are seeking a Post-Doctoral Fellow to work with an interactive collaborative team of scientists investigating the mechanisms of HIV-1 latency using CRISPR screens and validation in primary cell models of HIV latency. The applicant should be well organized, pay close attention to detail, be reliable and motivated to participate in multiple levels of lab activity.
Responsibilities
The fellow will perform standard routine and investigative laboratory work in support of scientific research specific to HIV latency and CRISPR screens. Major duties include:
- Perform experiments at the bench level to support investigative work, potentially including, but not limited to molecular biology, viral infections and assays, DNA sequencing, PCR amplification, RT-PCR amplification, TC culture, BLS2/3 work, including making virus stocks and performing viral infection
- CRISPR/Cas9 screening and editing, and analysis of results
- Conduct experiments with primary T cells
- Recover, compile and verify accuracy of research data
- Operate analytical instruments
- Perform and modify documented protocols and assays
- Maintain laboratory records and compile data on experiments and assays
- Interpret and report on experimental results
- Present data at local and national meetings
- Prepare manuscripts describing the data and their meaning to the field
Qualifications
Educational or Special Training Qualifications
- BA/BS required in a scientific field
- Applicants should have a PhD (or equivalent degree) in Biomedical Sciences and basic techniques in molecular biology
- Ability to think critically and design and interpret experiments is essential, as are strong communication skills
Experience Requirements
- Experience with molecular biology and biochemical methods
- A strong interest in research in the area of HIV biology and HIV latency, infectious diseases and global health
- Evidence of productivity appropriate to a postdoctoral fellow as measured by publications and presentations, abstracts and posters at conferences
- Well-organized with strong attention to detail
- Ability to work independently and perform experiments without day-to-day supervision will be required after training
- Ability to work as part of a team and to participate in multiple projects in collaboration with different lab members and collaborations with outside labs
Please include a cover letter with your application, decribing your research accomplishments as well as detailing your interest and suitability for this position.